ASTM D5465-93(2012) - 1.6.2012
 
Significance and Use

These practices provide a uniform set of counting, calculating, and reporting procedures for ASTM test methods in microbiology.

Section
ACounting Colonies on Membrane Filters4
BCounting Colonies on Pour Plates5
CCounting Colonies on Spread Plates6

The counting rules provide a best attainable estimate of microorganisms in the sample, since the samples cannot be held and reanalyzed at a later date.

 
1. Scope

1.1 These practices cover recommended procedures for counting colonies and reporting colony-forming units (CFU) on membrane filters (MF) and standard pour and spread plates.

1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

 
2. Referenced Documents

D5259-24

Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure

D1129-24

Standard Terminology Relating to Water

9215

Heterotrophic Plate Count Available online from http://standardmethods.org/store/ProductView.cfm?ProductID=102, or from American Public Health Association (APHA), Standard Methods for the Examination of Water and Wastewater, 800 I Street, NW Washington, DC 20001, http://www.apha.org.

E2563-23

Standard Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method

D6974-25

Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures

D6161-19

Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes

D5392-24

Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure