1.1 This test method covers a procedure for determining the degree of biodegradability of lubricants or their components in an aerobic aqueous medium on exposure to an inoculum under controlled laboratory conditions. This test method is an ultimate biodegradation test that measures oxygen demand in a closed respirometer.

1.2 This test method is suitable for evaluating the biodegradation of volatile as well as nonvolatile lubricants or lubricant components.

1.3 This test method is applicable to lubricants and lubricant components which are not toxic and not inhibitory to the test microorganisms at the test concentration.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazards are given in Section 10.