ASTM E1259-94 - 10.4.2001
 
1. Scope

1.1 This test method is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D 396, D 910, D 975, D 1655, D 2069, D 2880, D 3699, D 4818 and D 6227), system deterioration, or both.

1.2 Knowledge of microbiological techniques is required for these procedures.

1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

 
2. Referenced Documents

E1326-25

Standard Guide for Evaluating Non-culture Microbiological Tests

D7463-25

Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water

D7464-25

Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

D396-26a

Standard Specification for Fuel Oils (Includes all amendments and changes 4/14/2026).

Determining Corrosive Properties of Cargoes in Petroleum Product Pipelines Item No. 21204, available from NACE International (NACE), 1440 South Creek Dr., Houston, TX 77084-4906, http://www.nace.org.

40 CFR Part 152

Pesticide Registration and Classification Procedures

40 CFR Part 79

Fuels and Fuel Additives Registration Regulations Available from U.S. Government Printing Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401.

D910-26

Standard Specification for Leaded Aviation Gasolines

D6729-25

Standard Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 100 Metre Capillary High Resolution Gas Chromatography

D6469-24

Standard Guide for Microbial Contamination in Fuels and Fuel Systems

D6293-98

Standard Test Method for Oxygenates and Paraffin, Olefin, Naphthene, Aromatic (O-PONA) Hydrocarbon Types in Low-Olefin Spark Ignition Engine Fuels by Gas Chromatography

D6227-24a

Standard Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component (Includes all amendments and changes 12/11/2024).

D5465-25

Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

D4814-25a

Standard Specification for Automotive Spark-Ignition Engine Fuel (Includes all amendments and changes 12/24/2025).

D3699-25

Standard Specification for Kerosine

D2880-23

Standard Specification for Gas Turbine Fuel Oils

D2069-91(1998)

Standard Specification for Marine Fuels (Withdrawn 2003)

D1655-25a

Standard Specification for Aviation Turbine Fuels (Includes all amendments and changes 12/17/2025).

E1259-23

Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 ?C

D7978-25a

Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method (Includes all amendments and changes 7/30/2025).

D7687-23

Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration

D7467-23

Standard Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)

D6974-25

Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures

D975-26b

Standard Specification for Diesel Fuel (Includes all amendments and changes 3/31/2026).

D6733-24

Standard Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 50-Metre Capillary High Resolution Gas Chromatography

D6751-24

Standard Specification for Biodiesel Fuel Blendstock (B100) for Middle Distillate Fuels