This test method provides for the qualitative and quantitative determination of denatonium ion in engine coolant in milligrams per liter to low percent range and requires approximately 100 mL per test, with results available in less than 30 min. Acceptable levels of denatonium vary with manufacturer’s blending specifications and applicable minimum or maximum industry and state specifications. Typically specification ranges from 30 to 50 ppm denatonium ion.

1.1 This test method covers the chemical analysis of engine coolant for denatonium ion by high-performance liquid chromatography (HPLC).

1.2 This test method is applicable to both new and used coolants.

1.3 Coelution of other ions may cause interferences in the detection of the denatonium cation. In the case of unfamiliar formulations, identification verification should be performed by either or both fortification and dilution of the sample matrix with denatonium ion..

1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.