ASTM D7847-12 - 1.12.2012
 
1. Scope

1.1 Microbiological test methods present challenges that are unique relative to chemical or physical parameters, because microbes proliferate, die off and continue to be metabolically active in samples after those samples have been drawn from their source.

1.1.1 Microbial activity depends on the presence of available water. Consequently, the detection and quantification of microbial contamination in fuels and lubricants is made more complicated by the general absence of available water from these fluids.

1.1.2 Detectability depends on the physiological state and taxonomic profile of microbes in samples. These two parameters are affected by various factors that are discussed in this guide, and contribute to microbial data variability.

1.2 This guide addresses the unique considerations than must be accounted for in the design and execution of interlaboratory studies intended to determine the precision of microbiological test methods designed to quantify microbial contamination in fuels, lubricants and similar low water-content (water activity <0.8) fluids.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

 
2. Referenced Documents

IP 385

Viable aerobic microbial content of fuels and fuel components boiling below 90C--Filtration and culture method

E2756-24ae1

Standard Terminology Relating to Antimicrobial and Antiviral Agents (Includes all amendments and changes 10/30/2024).

E1601-19

Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method

D6300-26

Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants

D6469-24

Standard Guide for Microbial Contamination in Fuels and Fuel Systems

D6974-25

Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures

D7463-25

Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water

D7464-25

Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

D7687-23

Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration

E1326-25

Standard Guide for Evaluating Non-culture Microbiological Tests

D156-23

Standard Test Method for Saybolt Color of Petroleum Products (Saybolt Chromometer Method)

D1129-24

Standard Terminology Relating to Water

D4012-23a

Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water (Includes all amendments and changes 11/9/2023).

D4175-24e1

Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants (Includes all amendments and changes 11/19/2024).

E1259-23

Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 ?C