ASTM E1259-05

ASTM E1259-05 - 1.11.2005

Significance and Use

Guide D 6469 details the types of problems associated with uncontrolled microbial growth in fuels and fuel systems. Treatment with effective antimicrobial agents is one element of contamination control strategy.

The procedure should be used to evaluate the relative efficacy of microbicides in liquid fuels boiling below 390°C. The effect of environmental conditions, such as a variety of fuel additives, metal surfaces, and climatology, are variables that can be included in specific tests using this protocol.

This practice addresses product performance issues only. Regulatory Agencies restrict and control the use of both pesticides (in the U.S.: 40 CFR 152) and fuel additives (40 CFR 79). Regardless of performance in this method, antimicrobials must only be used in compliance with applicable regulations. Specific industries, for example, the aviation industry, may place further restrictions on chemicals used for fuel treatment.

1. Scope

1.1 This practice is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D 396, D 910, D 975, D 1655, D 2069, D 2880, D 3699, D 4814, D 6227, and D 6751), system deterioration, or both.

1.2 Knowledge of microbiological techniques is required for these procedures.

1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2. Referenced Documents

E1326-20	Standard Guide for Evaluating Non-culture Microbiological Tests
D7463-21	Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
D7464-20	Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
D396-21	Standard Specification for Fuel Oils
	Determining Corrosive Properties of Cargoes in Petroleum Product Pipelines Item No. 21204, available from NACE International (NACE), 1440 South Creek Dr., Houston, TX 77084-4906, http://www.nace.org.
40 CFR Part 152	Pesticide Registration and Classification Procedures
40 CFR Part 79	Fuels and Fuel Additives Registration Regulations Available from U.S. Government Printing Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401.
D910-24	Standard Specification for Leaded Aviation Gasolines
D6729-20	Standard Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 100 Metre Capillary High Resolution Gas Chromatography
D6469-20	Standard Guide for Microbial Contamination in Fuels and Fuel Systems
D6293-98	Standard Test Method for Oxygenates and Paraffin, Olefin, Naphthene, Aromatic (O-PONA) Hydrocarbon Types in Low-Olefin Spark Ignition Engine Fuels by Gas Chromatography
D6227-24	Standard Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component
D5465-16(2020)	Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
D4814-23a	Standard Specification for Automotive Spark-Ignition Engine Fuel (Includes all amendments and changes 12/14/2023).
D3699-19	Standard Specification for Kerosine
D2880-23	Standard Specification for Gas Turbine Fuel Oils
D2069-91(1998)	Standard Specification for Marine Fuels (Withdrawn 2003)
D1655-23a	Standard Specification for Aviation Turbine Fuels (Includes all amendments and changes 10/11/2023).
E1259-23	Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 ?C
D7978-14(2019)	Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water´Thixotropic Gel Culture Method
D7687-23	Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
D7467-23	Standard Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)
D6974-20	Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels´Filtration and Culture Procedures
D975-23a	Standard Specification for Diesel Fuel (Includes all amendments and changes 12/19/2023).
D6733-01(2020)	Standard Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 50-Metre Capillary High Resolution Gas Chromatography
D6751-23a	Standard Specification for Biodiesel Fuel Blendstock (B100) for Middle Distillate Fuels (Includes all amendments and changes 5/3/2023).