This test method provides a technique for evaluating antimicrobials in or on polymeric solids against staining by Streptoverticillium reticulum, and should assist in the prediction of performance of treated articles under actual field conditions.

Conditioning of the specimens, such as exposure to leaching, weathering, and heat treatment, may have significant effects on performance of antimicrobials against staining. Determination of these effects is not included in this test method.

1.1 This test method is used for determining the performance of antimicrobial agents used in or on synthetic polymeric solids against staining by the actinomycete Streptoverticillium reticulum. This organism has been chosen as an indicator organism, although other organisms have been known to cause undesirable staining in polymeric solids.

1.2 This test method is not suitable for evaluating dark-pigmented test samples.

1.3 A knowledge of microbiological techniques is recommended for these procedures.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.