ASTM E1841-96 - 10.11.1996
 
1. Scope

1.1 This test guide is designed to give general guidance for assessing the potential phytotoxicity of water soluble test material to freshwater emergent macrophytes.

1.2 This renewal test continuously exposes selected plant species, growing in sediment, to various concentrations of test material, dissolved in a nutrient solution.

1.3 This test guide is based on the Toxic Substances Control Act (TSCA) guidelines for conducting toxicity tests with terrestrial plants and is applicable to most water soluble chemicals , either individually or in formulations, commercial products, or known mixtures (see Guides E 1193 and E 1598). With slight modifications the procedure also might be used for effluents (see Guide E 1192).

1.4 Results from this toxicity test can be used to report an EC50 or NOEC (see Section 3) based on the concentration of chlorophyll extracted from the plants (see Guides D 3731 and E 1218). In some situations, it might be necessary to only test at one concentration to determine whether or not that specific concentration is toxic to the plants.

 
2. Referenced Documents

E943-23

Standard Terminology Relating to Biological Effects and Environmental Fate

E1733-22

Standard Guide for Use of Lighting in Laboratory Testing

E1706-20

Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates

E1598-94

Standard Practice for Conducting Early Seedling Growth Tests (Withdrawn 2003)

E1391-03(2023)

Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates

E1218-21

Standard Guide for Conducting Static Toxicity Tests with Microalgae

E1193-20

Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests

E1192-23

Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

E1023-23

Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

E729-23e1

Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians (Includes all amendments and changes 2/22/2023).

D3731-20

Standard Practices for Measurement of Chlorophyll Content of Algae in Surface Waters